TLC mfe ụzọ mgbagwoju analysis

TLC bụ onye ndú na okwu nke quantitative na ọkara quantitative analysis of mgbagwoju ọgwụ, eke, Biomedical, nkà na ụzụ, chemical na ọtụtụ ndị ọzọ bekee. Mkpa oyi akwa chromatography bụkwa ihe kasị inweta usoro nke uka analysis nke fọrọ nke nta niile na klas nke bekee.

Nke a na Usoro nke nkewa na analysis of multi-akụrụngwa usoro na-ọtụtụ-eji laboratories nke dị nnọọ iche specificity na direction: ịdị ọcha-epidemiological, ọgwụgwọ ọrịa anụmanụ, agrochemical, nnyocha, forensic, na n'ihi na osisi nchedo ụgbọ, Center maka Standardization na Bureau of Forensic Medicine.

Isi uru nke mkpa-oyi akwa chromatography, dabeere na ngagharị eluent (analyte mgbaze) na sorbent oyi akwa site na otú a na-akpọ capillary agha bụ mfe na easiness nke chromatographic usoro, akwa ogo nkenke nke analysis na dịtụ ala na-eri nke dị mkpa na akụrụngwa.

TLC, na-enwe a elu ogo uche (ala nchọpụta fọrọ) na selectivity, na-enye ohere ikpebi 10-20 ug bekee ruo 7%, nke bụ nnọọ elu. Mkpa oyi akwa chromatography nwere ike ịbụ adsorption na nkesa ụdị. The kasị n'ọtụtụ ebe bụ nke mbụ nhọrọ.

Ihe kachasi mkpa n'ime usoro idu ke TLC mgbaze umi Nkea na sorbent bed na gbasara ohere nkesa nke dị iche iche na mmiri dị ka ha na onye e ji mara na Njirimara. Eziokwu ahụ bụ na ụmụ irighiri ihe dị iche iche mejupụtara na Ọdịdị nke adsorbed (edebe) n'elu siri ike chromatographic efere dị iche iche. Ya mere, mgbagwoju multi-akụrụngwa usoro na-eji nwayọọ nwayọọ iche n'ime onye components.

Akpụ akpụ na dị iche iche gbapụrụ ọsọ tinyere sorbent bed, onye a na-ama iche, na mmiri na-etolite a chromatogram. Onye ọ bụla na agba ntụpọ kwekọrọ na a kpọmkwem chemical. Colorless umi na ultraviolet ìhè n'okpuru nduzi nke nke a plurality nke ogige amalite fluoresce, ma ọ bụ doro pụrụ iche ọgwụgwọ agba? Reagent.

N'etiti oge a gbasara nyocha ụzọ GLC guzo. Nke a na usoro e ji nkewa na analysis iji, dị ka a sorbing gị n'ụlọnga pụrụ iche nonvolatile mmiri mmiri, nke na powdered seramiiki ihe a na wetted. Na dị ka ndị na-akwọ ụgbọala na-ajụ na-eji ebe a dị iche iche inert, haruru. Usoro a bụ kasị dị irè maka ọmụmụ nke mgbagwoju mixtures na-ebu ọtụtụ narị dị iche iche mmiri.

Ọzọ oge a na usoro nke-ikpọ ndị mejupụtara nke mgbagwoju bekee bụ mmiri mmiri chromatography. The isi mma nke usoro a mejupụtara na nkewa nke onye mmiri site na iji iche iche na ha na-ekesa n'etiti ibughari (eluent) na ofu (adsorbent) n'ụzọ. Nke a na usoro a na-kasị na-eji na analysis nke dị iche iche organic ogige (nri nwekwara, pesticides, dị iche iche na toxins, wdg). Ọzọkwa, dị a chromatographic usoro a na-eji na-amụ àgwà akara nke na-emepụta ọgwụ na ngwaahịa, mkpebi siri ike nke Steroid na ndu oke mmiri, na ndị ọzọ na amino acid analysis.