Recombinant erythropoietin. Recombinant mmadụ erythropoietin

Erythropoietin (EPO) - glycoprotein hormone na akara ọnụego erythropoiesis mmadụ. The ugbu compound na-synthesized isi na akụrụ, a obere ego (banyere 10 percent) na-emepụta na imeju. The hormone erythropoietin activates nkewa na iche nke erythroid progenitors. Etoju nke endogenous hormone n'ime ọbara plasma nke ike ndị mmadụ n'otu n'otu dịgasị n'ime a dịgasị iche iche na bụ inversely proportional na haemoglobin ịta na ogo oxygenation nke anụ ahụ. Ọrụ na mwepu na mmepụta nke a hormone iji nkà mmụta ọgwụ bụ nnọọ uru.

Structure na uru hormone

The molekul mejupụtara amino asịd hormone. Na erughi nke endogenous erythropoietin kemgbe a nkọ ọnụ ke larịị nke haemoglobin na mkpụrụ ndụ ọbara uhie, ọ na-emepe emepe na-akpọ eritropoetindefitsitnaya anaemia. nọgidere-agaghị ekwe omume n'ihi enweghị kwesịrị ekwesị na-emepụta ọgwụ Ruo mgbe na-adịbeghị anya, ndị dị otú ahụ a ọgwụ mgbazi nke anaemia. Taa, na a mpe n'elu hormone na ahụ mmadụ, dọkịta nye iwu recombinant erythropoietin. The nkwadebe enwetara site na anụmanụ mkpụrụ ndụ nke na ẹkenam site n'usoro koodu n'ihi na mmadụ EPO. Recombinant mmadụ erythropoietin na amino acid na carbohydrate mejupụtara bụ yiri eke hormone, enwekwu ọnụ ọgụgụ nke mkpụrụ ndụ ọbara uhie, reticulocytes, activates biosynthesis haemoglobin na mkpụrụ ndụ. The ndu ọrụ nke dapụtara ihe onwunwe ọ dị iche iche si endogenous hormone. Recombinant erythropoietin anaghị igosipụta cytotoxic mmetụta na-adịghị emetụta leucopoiesis. Ọkà mmụta sayensị na-atụ aro na EPO interacts na kpọmkwem eritropoetinchuvstvitelnymi anabata, nke na-emi odude na cell n'elu.

A usoro maka ọcha recombinant mmadụ erythropoietin

Recombinant mmadụ EPO - bụ otu n'ime ihe ndị kasị ukwuu na-edozi na-emepụta site na ọtụtụ ndu na-emepụta ọgwụ na ụlọ ọrụ ndị na gburugburu ụwa maka aṅụ ọgwụ. The ugbu compound na-synthesized ovary mkpụrụ ndụ Chinese Hamster (Cho) site recombinant DNA usoro. Otu polypeptide yinye recombinant EPO nwere 165 amino asịd, na gbakọọ molekụla arọ nke 24,000 Yeah, na hụrụ molekụla arọ nke glycosylated protein - 30 400 Da. Alaka erythropoietin si adịghị rụrụ site ion-mgbanwe na mmekọrita chromatography. Human recombinant EPO nwere a na-adị ọcha nke 98%.

Recombinant erythropoietin na analogs ya

Iji kpalie erythropoiesis usoro dọkịta eji dị iche iche ọgwụ ọjọọ eme ihe:

• "Aranesp";
• "Aeprin";
• "Epobiokrin";
• "Bioein";
• "Vepoks";
• "Binokrit";
• "Epokrin";
• "Gemax";
• "Epogen";
• "Eprex";
• "Epovitan";
• "Epomaks";
• "Giperkrit";
• "Eralfon";
• "Eritrea";
• "Recormon";
• "Epostim";
• "Epozino";
• "Epoetin Beta".

Tupu ị na-eji dochie a recombinant erythropoietin analogues, kwesịrị ịga hụ dọkịta.

egosi

Medical practitioners mgbe obibi eritropoetindefitsitnymi anaemia (Eda). Nke a na-agụnye ndị na-esonụ pathologies:

• anaemia nke ịza neoplasms;
• n'oge anaemia nke prematurity amụ ije (tupu 34 izu gestation) na a arọ ahụ nke 750 1500 g;
• nephrogenic anaemia;
• Anaemia na-adịghị ala ala ọrịa na (ịba ọcha n'anya C, ọrịa ogbu na nkwonkwo, HIV ọrịa, alimentary kanaal ọrịa).

A akụkụ pụrụ iche nke n'elu-erughi anaemia bụ eziokwu na ha na-adịghị emeso ígwè preparations. Ruo mgbe na-adịbeghị anya, nanị irè ọgwụ bụ nke mmịnye ọbara. N'ihi na eziokwu na ụdị ọgwụ nwere a nnukwu ọnụ ọgụgụ nke mmetụta na a elu n'ihe ize ndụ nke nnyefe nke n'ọbara pathogens nke dị ize ndụ na-efe efe (HIV, ịba ọcha n'anya na nje virus, wdg), usoro a na nkà mmụta ọgwụ ọgbara na-fọrọ nke nta mgbe eji. Recombinant mmadụ erythropoietin na-enyere aka mma ndụ maka ọrịa na a nchoputa nke "eritropoetindefitsitnaya anaemia." Biosynthesis na iwebata recombinant mmadụ EPO meghere a n'ọgbọ ọhụrụ na ọgwụgwọ nke anaemia eritropoetinzavisimyh.

contraindications

Dọkịta adịghị ike ikwu na ojiji nke recombinant erythropoietin (ntuziaka adọ aka ná ntị banyere ya) na ọnụnọ nke ndị na-esonụ pathologies:

• thromboembolism;
• hypersensitivity ọgwụ;
• na-apụghị ime nke irè anticoagulant ọgwụ;
• agafere ụbụrụ ọrịa strok ma ọ bụ myocardial infarction;
• ejighị n'aka rịaworo;
• n'echeghị echiche ọbara mgbali;
• oge ịmụ nwa, nwa na ara;
• refractory ọbara mgbali.

dosing Usoro

The onunu ogwu na oge nke ọgwụgwọ atụmatụ na-guzosie ike a nditịm n'otu n'otu, dabere na ogo nke anaemia, izugbe ọnọdụ nke onye ọrịa ahụ na ọdịdị nke daa ọrịa. The ọgwụ na-zubere maka parenteral were. Mbụ doses nwere ike ịdị iche iche ke nso site na 50 ruo 150 IU / n'arọ. The dose kwesịrị ịgbanwe dabere na ọrịa afọ. Recombinant erythropoietin na-emekarị ndinọ 3 ugboro a izu. Ọ bụrụ na nnyefere e amụba mmetụta. N'ihi-aghọ ndị ọzọ ma ọ bụ obere anya mgbe 2-3 izu nke ngwa.

n'akụkụ mmetụta

Ị kara recombinant mmadụ erythropoietin? Ntuziaka maka Iji anaghị ewepu mmepe nke mmetụta. Ha bụ:

• myalgia;
• dizziness;
• hyperthermia;
• iro ụra;
• vomiting;
• isi ọwụwa;
• afọ ọsịsa;
• arthralgia;
• ọbara mgbali;
• mgbu;
• thrombocytosis;
• tachycardia;
• hypertensive nsogbu;
• ụfụ;
• steatosis;
• azụ larịị nke AST, Alt na ọbara;
• erythrocyte izizi cell aplasia;
• eczema;
• angioedema;
• akpụkpọ ọkụ ọkụ na itching;
• asthenia;
• aṅụ, nācha ọbara-ọbara na ọkụ na saịtị nke ochichi;
• hyperkalemia;
• Mbelata protein ịta na plasma ferritin;
• hyperphosphatemia.

Erythropoiesis stimulants na egwuregwu

Nso nso a, ugboro ugboro ji ọgwụ ọjọọ, "Epovitan" (recombinant mmadụ erythropoietin). The ugbu ngwá ọrụ a na-eji na egwuregwu (egwuregwu, bodybuilding, igwu mmiri, na biathlon). Sị na nkwadebe activates biosynthesis nke mkpụrụ ndụ ọbara uhie, nke n'aka nke na-eduga onye na-abawanye na ọdịnaya nke oxygen kwa unit olu nke ọbara na, mmọdo, ka onye na-abawanye na ọbara oxygen ike na O ₂ nnyefe ka akụkụ na anụ ahụ. A na usoro nke edinam enwekwu aerobic ntachi obi nke ihe na-eme egwuregwu. A mmetụta yiri na-hụrụ mgbe na-azụ ihe na-eme egwuregwu na a Midlands, ebe enweghị O ₂ na ikuku na-akpata mmepe nke hypoxia, nke n'aka nke activates biosynthesis nke endogenous erythropoietin. EPO preparations na-eji na Nchikota na insulin, ibu hormone (GH, somatotropin) na stanazolol.

Oké, agụụ iji "erythropoietin" ọgwụ ọjọọ na egwuregwu nwere ike ime ka ọbara na-egbochi mkpụkọ nke na-emekarị-eduba n'ọnwụ. Ịchọpụta recombinant EPO bụ nnọọ ihe siri ike, n'ihi na sịntetik ogige Ọdịdị bụ yiri ya n'ahụ analọg, otú a biosoedinenie ka iwu na-akwadoghị-eji na elu-arụmọrụ egwuregwu dị ka doping.